Overview of Peptide Synthesis

• Introduction
  
• Solid Phase Peptide Synthesis (SPPS)
  
• Fmoc Strategy in SPPS
  
• Boc Strategy in SPPS
  
• General Coupling Methods in SPPS
  
• Solution Phase Synthesis
  
• HPLC Analysis and Purification
  
• Handling and Storage of Peptides
  
• References
  
  

Tips for Peptide Synthesis

• Reconstitution and Storage of Peptides
  
• Methods to Dissolve Peptides
  
• Preparation of HBTU/HOBt Solution for the Peptide Synthesizer
  
• Biotinylation of Amino Group
  
• Procedure for Loading Fmoc-Amino Acid to 2-Chlorotrityl Chloride Resin
• Procedure for Checking Substitution of Fmoc-Amino Acid Loaded Resins
  
• Manual Fmoc Synthesis (0.25 mmol)
• Synthesis of Phosphotyrosine-Containing Peptides Using Fmoc-Phosphotyrosine
  
• Simultaneous Synthesis of Peptides Which Differ in the C-Termini Using 2-Chlorotrityl Resin and Wang Resin
  
• Cleavage Protocol to Produce Fully Protected Peptide
  
• Procedure for Removing Mtt group from Fmoc-Lys(Mtt) on Solid Phase
  
• Procedure for FITC Labeling of Peptides
  
• Procedure for Fluorescein Labeling of Peptides